However, strategies for the prospective isolation of cell populations newly identified by single-cell genomics are needed to enable their functional characterization or therapeutic use.
The systematic construction of whole-organ and whole-organism single-cell atlases has revealed an unanticipated diversity of cell types and cell states, and has provided detailed insights into cellular development and differentiation processes 4, 5, 6, 7. Single-cell transcriptomic technologies have revolutionized our understanding of tissues 1, 2, 3. Our study serves as an accessible resource and paves the way for a data-driven era in cytometry.
The systematic integration of cytometry and proteo-genomic data enables the functional capacities of precisely mapped cell states to be measured at the single-cell level. These reference maps enable the automatic design of cost-effective high-throughput cytometry schemes that outperform state-of-the-art approaches, accurately reflect complex topologies of cellular systems and permit the purification of precisely defined cell states. Here, we have generated high-content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all main hematopoietic cell types in healthy aging and leukemia. However, excessive cost and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Single-cell genomics technology has transformed our understanding of complex cellular systems. Nature Immunology volume 22, pages 1577–1589 ( 2021) Cite this article Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states
0 kommentar(er)
